FSH isoforms, radioimmunoassays, bioassays, and their significance.
نویسندگان
چکیده
Follicle-stimulating hormone (FSH) plays a central role in steroidogenesis and gametogenesis. In recent years, a great deal has been learned about the microheterogeneity of FSH using newly developed assay techniques. This review article will attempt to discuss the advantages and the limitations of recently developed and commonly used assay systems.
منابع مشابه
Bioactive Follicle-Stimulating Hormone
Specific and sensitive in vitro bioassays suitable for measurement of follicle-stimulating hormone (FSH) in small quantities of serum and urine have recently been validated. Comparisons of bioassay (B-FSH) potencies with immunoassay (I-FSH) estimates and chromatofocusing patterns have facilitated new discoveries regarding the regulation of FSH biosynthesis, storage, secretion, serum concentrati...
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In the present study, we analysed and compared the relative in-vitro biological activity of the various intrapituitary human follicle stimulating hormone (FSH) isoforms employing two different bioassay systems. FSH was fractionated by chromatofocusing (pH range 7.10 to < 3.80) and the several isoforms isolated were quantified at multiple dose levels by three highly specific immunoassay systems:...
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متن کاملValidation of highly specific and sensitive radioimmunoassays for lutropin, follitropin, and free alpha subunit in unextracted urine.
Measurement of the urinary excretion of lutropin (LH) and follitropin (FSH) and their common free alpha subunit (FAS) assists in monitoring the maturation of the hypothalamic-pituitary-gonadal axis and in understanding the physiology of the pituitary glycoprotein hormones. Here we describe sensitive, specific polyclonal radioimmunoassays for LH and FSH and a monoclonal radioimmunoassay for FAS ...
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FSH is synthesized and secreted by the anterior pituitary gland in multiple molecular forms; the release of these isoforms depends on the endocrine status of the donor at the time of sample collection. In the present study, we analysed the possibility that the FSH charge isoforms may exert differential effects at the target cell. Seven FSH isoform mixes were isolated from pooled anterior pituit...
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ورودعنوان ژورنال:
- Journal of andrology
دوره 13 1 شماره
صفحات -
تاریخ انتشار 1992